miscript pcr starter kit Search Results


miscript pcr starter kit  (Qiagen)
90
Qiagen miscript pcr starter kit
Miscript Pcr Starter Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/miscript pcr starter kit/product/Qiagen
Average 90 stars, based on 1 article reviews
miscript pcr starter kit - by Bioz Stars, 2026-02
90/100 stars
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miscript pcr starter kit # 2181193  (Qiagen)
90
Qiagen miscript pcr starter kit # 2181193
Miscript Pcr Starter Kit # 2181193, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/miscript pcr starter kit # 2181193/product/Qiagen
Average 90 stars, based on 1 article reviews
miscript pcr starter kit # 2181193 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
mirna quantification miscript pcr starter kit  (Qiagen)
90
Qiagen mirna quantification miscript pcr starter kit
a Representative confocal images of enteroids harvested from wild-type (WT) and AHR knockout ( Ahr -/- ) mice after 7 days of culture on Matrigel, and stained with the epithelial marker (Ecadherin, Ecad, green signal), actin filaments (Rhodamine Phalloidin, RP, red signal), and nuclei (DAPI, blue signal). b , <t>c</t> <t>qRT-PCR</t> showing the expression of Cyp1a1 ( b , n = 3, 3, 3, 3 wells of enteroids, WT -I3C vs WT + I3C p = 0.0208) and Tnf-α ( c , n = 3, 3, 3, 3 wells of enteroids, Ctrl vs WT LPS p < 0.0001, WT LPS vs WT LPS + I3C p = 0.0258) in these enteroids under the indicated condition (200 μM I3C pretreatment overnight and then LPS treatment (50 μg per mL) for 4 h). d , e Representative confocal images ( d ) and quantification of fluorescent intensity of NF-κB translocation ( e , n = 81, 48, 48, 50 enteroid cells, Ctrl -I3C vs LPS -I3C p < 0.0001, LPS -I3C vs LPS + I3C p < 0.0001). NF-κB, green signal; actin filaments (Rhodamine Phalloidin, RP, red signal); nuclei (DAPI, blue signal). f – i qRT-PCR showing the expression of Cyp1a1 ( f , n = 6, 8, 9, 8 mice, WT -I3C vs WT + I3C p = 0.0042), Il6 ( g , n = 6, 7, 8, 8, 8 mice, Ctrl vs WT LPS p = 0.0018, WT LPS vs WT LPS + I3C p = 0.0065), Tnf-α ( h , n = 6, 7, 8, 8, 8 mice, Ctrl vs WT LPS p = 0.0036, WT LPS vs WT LPS + I3C p = 0.0017) and Tlr4 ( i , n = 18, 19, 9, 8 mice, WT -I3C vs Ahr -/- -I3C p = 0.0013, WT -I3C vs WT + I3C p = 0.0033) in the ileum of newborn mice after treatment with or without I3C (25 mg per kg body weight per day for 4 days). j , k qRT-PCR showing the expression of the Tlr4 regulatory <t>microRNAs,</t> miR-146b ( j , n = 7, 5, 8, 5 mice WT -I3C vs WT + I3C p = 0.0300), let-7i ( k , n = 6, 5, 7, 5 mice WT -I3C vs WT + I3C p = 0.0016) and miR-223 ( l , n = 7, 5, 8, 5 mice WT -I3C vs WT + I3C p < 0.0001) in the ileum of WT and Ahr -/- mice in the presence or absence of I3C (25 mg per kg body weight for 24 h). m , n qRT-PCR showing expression of CYP1A1 ( m , n = 3, 3, 3, 3 wells of human explant culture, Ctrl -I3C vs Ctrl +I3C p = 0.0147, LPS -I3C vs LPS + I3C p = 0.0096) and TNF-α ( n , n = 3, 3, 3, 3 wells of human explant culture, Ctrl -I3C vs LPS -I3C p = 0.0003, LPS -I3C vs LPS + I3C p = 0.0218) in the presence of I3C (200 μM I3C pretreatment for 15 min and then additional 6 h) and LPS (50 μg per mL for 6 h). Scale bars in a , 25 μm. Scale bars in d , 10 μm. All data are presented as mean values ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, p values obtained either from two-sided t tests or one-way ANOVA followed by multiple comparisons. Each dot in graphs represents data from an individual well of enteroids culture, an individual enteorid cell of NF-κB staining, an individual mouse, or an individual well of human explant culture.
Mirna Quantification Miscript Pcr Starter Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mirna quantification miscript pcr starter kit/product/Qiagen
Average 90 stars, based on 1 article reviews
mirna quantification miscript pcr starter kit - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


a Representative confocal images of enteroids harvested from wild-type (WT) and AHR knockout ( Ahr -/- ) mice after 7 days of culture on Matrigel, and stained with the epithelial marker (Ecadherin, Ecad, green signal), actin filaments (Rhodamine Phalloidin, RP, red signal), and nuclei (DAPI, blue signal). b , c qRT-PCR showing the expression of Cyp1a1 ( b , n = 3, 3, 3, 3 wells of enteroids, WT -I3C vs WT + I3C p = 0.0208) and Tnf-α ( c , n = 3, 3, 3, 3 wells of enteroids, Ctrl vs WT LPS p < 0.0001, WT LPS vs WT LPS + I3C p = 0.0258) in these enteroids under the indicated condition (200 μM I3C pretreatment overnight and then LPS treatment (50 μg per mL) for 4 h). d , e Representative confocal images ( d ) and quantification of fluorescent intensity of NF-κB translocation ( e , n = 81, 48, 48, 50 enteroid cells, Ctrl -I3C vs LPS -I3C p < 0.0001, LPS -I3C vs LPS + I3C p < 0.0001). NF-κB, green signal; actin filaments (Rhodamine Phalloidin, RP, red signal); nuclei (DAPI, blue signal). f – i qRT-PCR showing the expression of Cyp1a1 ( f , n = 6, 8, 9, 8 mice, WT -I3C vs WT + I3C p = 0.0042), Il6 ( g , n = 6, 7, 8, 8, 8 mice, Ctrl vs WT LPS p = 0.0018, WT LPS vs WT LPS + I3C p = 0.0065), Tnf-α ( h , n = 6, 7, 8, 8, 8 mice, Ctrl vs WT LPS p = 0.0036, WT LPS vs WT LPS + I3C p = 0.0017) and Tlr4 ( i , n = 18, 19, 9, 8 mice, WT -I3C vs Ahr -/- -I3C p = 0.0013, WT -I3C vs WT + I3C p = 0.0033) in the ileum of newborn mice after treatment with or without I3C (25 mg per kg body weight per day for 4 days). j , k qRT-PCR showing the expression of the Tlr4 regulatory microRNAs, miR-146b ( j , n = 7, 5, 8, 5 mice WT -I3C vs WT + I3C p = 0.0300), let-7i ( k , n = 6, 5, 7, 5 mice WT -I3C vs WT + I3C p = 0.0016) and miR-223 ( l , n = 7, 5, 8, 5 mice WT -I3C vs WT + I3C p < 0.0001) in the ileum of WT and Ahr -/- mice in the presence or absence of I3C (25 mg per kg body weight for 24 h). m , n qRT-PCR showing expression of CYP1A1 ( m , n = 3, 3, 3, 3 wells of human explant culture, Ctrl -I3C vs Ctrl +I3C p = 0.0147, LPS -I3C vs LPS + I3C p = 0.0096) and TNF-α ( n , n = 3, 3, 3, 3 wells of human explant culture, Ctrl -I3C vs LPS -I3C p = 0.0003, LPS -I3C vs LPS + I3C p = 0.0218) in the presence of I3C (200 μM I3C pretreatment for 15 min and then additional 6 h) and LPS (50 μg per mL for 6 h). Scale bars in a , 25 μm. Scale bars in d , 10 μm. All data are presented as mean values ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, p values obtained either from two-sided t tests or one-way ANOVA followed by multiple comparisons. Each dot in graphs represents data from an individual well of enteroids culture, an individual enteorid cell of NF-κB staining, an individual mouse, or an individual well of human explant culture.

Journal: Nature Communications

Article Title: Maternal aryl hydrocarbon receptor activation protects newborns against necrotizing enterocolitis

doi: 10.1038/s41467-021-21356-4

Figure Lengend Snippet: a Representative confocal images of enteroids harvested from wild-type (WT) and AHR knockout ( Ahr -/- ) mice after 7 days of culture on Matrigel, and stained with the epithelial marker (Ecadherin, Ecad, green signal), actin filaments (Rhodamine Phalloidin, RP, red signal), and nuclei (DAPI, blue signal). b , c qRT-PCR showing the expression of Cyp1a1 ( b , n = 3, 3, 3, 3 wells of enteroids, WT -I3C vs WT + I3C p = 0.0208) and Tnf-α ( c , n = 3, 3, 3, 3 wells of enteroids, Ctrl vs WT LPS p < 0.0001, WT LPS vs WT LPS + I3C p = 0.0258) in these enteroids under the indicated condition (200 μM I3C pretreatment overnight and then LPS treatment (50 μg per mL) for 4 h). d , e Representative confocal images ( d ) and quantification of fluorescent intensity of NF-κB translocation ( e , n = 81, 48, 48, 50 enteroid cells, Ctrl -I3C vs LPS -I3C p < 0.0001, LPS -I3C vs LPS + I3C p < 0.0001). NF-κB, green signal; actin filaments (Rhodamine Phalloidin, RP, red signal); nuclei (DAPI, blue signal). f – i qRT-PCR showing the expression of Cyp1a1 ( f , n = 6, 8, 9, 8 mice, WT -I3C vs WT + I3C p = 0.0042), Il6 ( g , n = 6, 7, 8, 8, 8 mice, Ctrl vs WT LPS p = 0.0018, WT LPS vs WT LPS + I3C p = 0.0065), Tnf-α ( h , n = 6, 7, 8, 8, 8 mice, Ctrl vs WT LPS p = 0.0036, WT LPS vs WT LPS + I3C p = 0.0017) and Tlr4 ( i , n = 18, 19, 9, 8 mice, WT -I3C vs Ahr -/- -I3C p = 0.0013, WT -I3C vs WT + I3C p = 0.0033) in the ileum of newborn mice after treatment with or without I3C (25 mg per kg body weight per day for 4 days). j , k qRT-PCR showing the expression of the Tlr4 regulatory microRNAs, miR-146b ( j , n = 7, 5, 8, 5 mice WT -I3C vs WT + I3C p = 0.0300), let-7i ( k , n = 6, 5, 7, 5 mice WT -I3C vs WT + I3C p = 0.0016) and miR-223 ( l , n = 7, 5, 8, 5 mice WT -I3C vs WT + I3C p < 0.0001) in the ileum of WT and Ahr -/- mice in the presence or absence of I3C (25 mg per kg body weight for 24 h). m , n qRT-PCR showing expression of CYP1A1 ( m , n = 3, 3, 3, 3 wells of human explant culture, Ctrl -I3C vs Ctrl +I3C p = 0.0147, LPS -I3C vs LPS + I3C p = 0.0096) and TNF-α ( n , n = 3, 3, 3, 3 wells of human explant culture, Ctrl -I3C vs LPS -I3C p = 0.0003, LPS -I3C vs LPS + I3C p = 0.0218) in the presence of I3C (200 μM I3C pretreatment for 15 min and then additional 6 h) and LPS (50 μg per mL for 6 h). Scale bars in a , 25 μm. Scale bars in d , 10 μm. All data are presented as mean values ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, p values obtained either from two-sided t tests or one-way ANOVA followed by multiple comparisons. Each dot in graphs represents data from an individual well of enteroids culture, an individual enteorid cell of NF-κB staining, an individual mouse, or an individual well of human explant culture.

Article Snippet: For microRNA isolation, freshly harvested, snap-frozen ileal tissue was subjected to total RNA isolation using the RNeasy plus universal mini kit (Qiagen) and miRNA was quantified using miScript PCR starter kit (Qiagen) following the manufacturer’s protocols.

Techniques: Knock-Out, Staining, Marker, Quantitative RT-PCR, Expressing, Translocation Assay